A simple and rapid nonviral approach to efficiently transfect primary tissue–derived cells using polyethylenimine
Author:
Publisher
Springer Science and Business Media LLC
Subject
General Biochemistry, Genetics and Molecular Biology
Link
https://www.nature.com/articles/nprot.2012.038.pdf
Reference39 articles.
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2. Akinc, A., Thomas, M., Klibanov, A.M. & Langer, R. Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis. J. Gene Med. 7, 657–663 (2005).
3. Wightman, L. et al. Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo. J. Gene Med. 3, 362–372 (2001).
4. Rejman, J., Oberle, V., Zuhorn, I.S. & Hoekstra, D. Size-dependent internalization of particles via the pathways of clathrin- and caveolae-mediated endocytosis. Biochem. J. 377, 159–169 (2004).
5. Gersdorff von, K. et al. The internalization route resulting in successful gene expression depends on both cell line and polyethylenimine polyplex type. Mol. Ther. 14, 745–753 (2006).
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