Abstract
AbstractBenefits of whole grains as dietary supplements and active ingredients in health products have been promoted. Despite being neglected as an agricultural byproduct of polished rice, pigmented rice bran has emerged as a promising source of natural anti-aging compounds. Indeed, the extract of red rice bran Hom Dang cultivar contained rich phenolic acids and flavonoids. It displayed high antioxidant activities in vitro and in vivo assays. Using yeast model, extract and bioactive compounds, quercetin and protocatechuic acid found in the rice bran pericarp, effectively reduced levels of intracellular reactive oxygen species (ROS), restored plasma membrane damages and prolonged life-span of pre-treated wild-yeast cells. Importantly, these molecules modulated life span-extension through a mechanism of ROS reduction that resembles to that operated under the highly conserved Tor1- and Sir2-dependent signaling pathways, with the human homologs TORC1 and SIRT1, respectively. The key longevity factors Sch9 and Rim15 kinases, Msn2/4 regulators and a novel transcription factor Asg1, the antioxidant enzymes superoxide dismutases and glutathione peroxidases played important role in mediating longevity. Yeast clearly provides an instrumental platform for rapid screening of compounds with anti-aging efficacies and advances knowledge in the molecular study of ageing.
Funder
National Research Council of Thailand
King Mongkut's University of Technology Thonburi
Petchra Pra Jom Klao Scholarship
Publisher
Springer Science and Business Media LLC
Reference63 articles.
1. Botstein, D., Chervitz, S. A. & Cherry, J. M. Yeast as a model organism. Science. 277, 1259–1260 (1997).
2. Longo, V. D. & Fabrizio, P. Chronological aging in Saccharomyces cerevisiae. Subcell Biochem. 57, 101–121 (2012).
3. Canakci, C. F., Cicek, Y. & Canakci, V. Reactive oxygen species and human inflammatory periodontal diseases. Biochemistry (Mosc). 70, 619–628 (2005).
4. Valko, M. et al. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 39, 44–84 (2007).
5. Devasagayam, T. P. et al. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physicians India. 52, 794–804 (2004).
Cited by
20 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献