Author:
Malheiros Jessica Moraes,Correia Banny Silva Barbosa,Ceribeli Caroline,Cardoso Daniel Rodrigues,Colnago Luiz Alberto,Junior Stanislau Bogusz,Reecy James Mark,Mourão Gerson Barreto,Coutinho Luiz Lehmann,Palhares Julio Cesar Pascale,Berndt Alexandre,de Almeida Regitano Luciana Correia
Abstract
AbstractWe conducted a study to identify the fecal metabolite profile and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fluid were collected before and at slaughter, respectively. The metabolomics analysis indicated 49 common polar metabolites in the rumen and feces. Acetate, propionate, and butyrate were the most abundant polar metabolites in both bio-samples. The rumen presented significantly higher concentrations of the polar compounds when compared to feces (P < 0.05); even though, fecal metabolites presented an accentuated representability of the ruminal fluid metabolites. All fatty acids present in the ruminal fluid were also observed in the feces, except for C20:2n6 and C20:4n6. The identified metabolites offer information on the main metabolic pathways (higher impact factor and P < 0.05), as synthesis and degradation of ketone bodies; the alanine, aspartate and glutamate metabolisms, the glycine, serine; and threonine metabolism and the pyruvate metabolism. The findings reported herein on the close relationship between the ruminal fluid and feces metabolic profiles may offer new metabolic information, in addition to facilitating the sampling for metabolism investigation in animal production and health routines.
Funder
Fundação de Amparo à Pesquisa do Estado de São Paulo
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Publisher
Springer Science and Business Media LLC
Reference66 articles.
1. USDA. (2020). Livestock and Poultry: World Markets and Trade. In United States Department of Agriculture and Foreign Agricultural Service. http://apps.fas.usda.gov
2. Carvalho, M. E. et al. Heat shock and structural proteins associated with meat tenderness in Nellore beef cattle, a Bos indicus breed. Meat Sci. 96, 1318–1324 (2014).
3. Cônsolo, N. R. B., Ferrari, V. B., Mesquita, L. G., Goulart, R. S. & Silva, L. F. P. Zilpaterol hydrochloride improves beef yield, changes palatability traits, and increases calpain-calpastatin gene expression in Nellore heifers. Meat Sci. 121, 375–381 (2016).
4. Hobson, P. N., Wallace, R. J. & Bryant, M. P. Microbial ecology and activities in the rumen: Part II. Crit. Rev. Microbiol. 9, 253–320 (1982).
5. Harfoot, C. G. & Hazlewood, G. P. Lipid metabolism in the rumen. In The Rumen Microbial Ecosystem 2nd edn (eds Hobson, P. N. & Stewart, C. S.) 382–426 (Chapman & Hall, 1997).
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献