Author:
Herbig Maik,Jacobi Angela,Wobus Manja,Weidner Heike,Mies Anna,Kräter Martin,Otto Oliver,Thiede Christian,Weickert Marie‑Theresa,Götze Katharina S.,Rauner Martina,Hofbauer Lorenz C.,Bornhäuser Martin,Guck Jochen,Ader Marius,Platzbecker Uwe,Balaian Ekaterina
Abstract
AbstractDiagnosis of myelodysplastic syndrome (MDS) mainly relies on a manual assessment of the peripheral blood and bone marrow cell morphology. The WHO guidelines suggest a visual screening of 200 to 500 cells which inevitably turns the assessor blind to rare cell populations and leads to low reproducibility. Moreover, the human eye is not suited to detect shifts of cellular properties of entire populations. Hence, quantitative image analysis could improve the accuracy and reproducibility of MDS diagnosis. We used real-time deformability cytometry (RT-DC) to measure bone marrow biopsy samples of MDS patients and age-matched healthy individuals. RT-DC is a high-throughput (1000 cells/s) imaging flow cytometer capable of recording morphological and mechanical properties of single cells. Properties of single cells were quantified using automated image analysis, and machine learning was employed to discover morpho-mechanical patterns in thousands of individual cells that allow to distinguish healthy vs. MDS samples. We found that distribution properties of cell sizes differ between healthy and MDS, with MDS showing a narrower distribution of cell sizes. Furthermore, we found a strong correlation between the mechanical properties of cells and the number of disease-determining mutations, inaccessible with current diagnostic approaches. Hence, machine-learning assisted RT-DC could be a promising tool to automate sample analysis to assist experts during diagnosis or provide a scalable solution for MDS diagnosis to regions lacking sufficient medical experts.
Funder
Deutsche Forschungsgemeinschaft
DKMS Mechthild Harf Research Grant
Bundesministerium für Bildung und Forschung
German Jose Carreras Leukämiestiftung
Alfred & Angelika Gutermuth-Stiftung
Collaborative Research Center 655
Universitätsklinikum Carl Gustav Carus Dresden an der Technischen Universität Dresden
Publisher
Springer Science and Business Media LLC
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献