Optofluidic time‐stretch imaging flow cytometry with a real‐time storage rate beyond 5.9 GB/s

Author:

Hou Dan1,Zhou Jiehua1ORCID,Xiao Ruidong1,Yang Kaining1,Ding Yan1,Wang Du1,Wu Guoqiang1,Lei Cheng123

Affiliation:

1. The Institute of Technological Sciences Wuhan University Wuhan China

2. Shenzhen Institute of Wuhan University Shenzhen China

3. Suzhou Institute of Wuhan University Suzhou China

Abstract

AbstractOptofluidic time‐stretch imaging flow cytometry (OTS‐IFC) provides a suitable solution for high‐precision cell analysis and high‐sensitivity detection of rare cells due to its high‐throughput and continuous image acquisition. However, transferring and storing continuous big data streams remains a challenge. In this study, we designed a high‐speed streaming storage strategy to store OTS‐IFC data in real‐time, overcoming the imbalance between the fast generation speed in the data acquisition and processing subsystem and the comparatively slower storage speed in the transmission and storage subsystem. This strategy, utilizing an asynchronous buffer structure built on the producer‐consumer model, optimizes memory usage for enhanced data throughput and stability. We evaluated the storage performance of the high‐speed streaming storage strategy in ultra‐large‐scale blood cell imaging on a common commercial device. The experimental results show that it can provide a continuous data throughput of up to 5891 MB/s.

Funder

National Key Research and Development Program of China

Science Fund for Distinguished Young Scholars of Hubei Province

Publisher

Wiley

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