Abstract
AbstractDue to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 × 10−6 ng/mL in 20 s, with a wide linear range of 10−5–10−1 ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine.
Funder
National Natural Science Foundation of China
Publisher
Springer Science and Business Media LLC
Subject
Public Health, Environmental and Occupational Health,Food Science
Reference44 articles.
1. Bachofen, C. Selected viruses detected on and in our food. Curr. Clin. Microbiol. Rep. 5, 143–153 (2018).
2. Severi, E. et al. Large and prolonged food-borne multistate hepatitis A outbreak in Europe associated with consumption of frozen berries, 2013 to 2014. Eur. Surveill. 20, 21192 (2015).
3. Young, K. Y. et al. Epidemiological and genetic analysis of a sustained communitywide outbreak of hepatitis A in the Republic of Korea, 2008: a hospital-based case-control study. J. Clin. Virol. 46, 184–188 (2009).
4. Hall, A. J. Noroviruses: the perfect human pathogens? J. Infect. Dis. 205, 1622–1624 (2012).
5. Somura, Y. et al. Detection of Norovirus in food samples collected during suspected food-handler-involved foodborne outbreaks in Tokyo. Lett. Appl. Microbiol. 69, 175–180 (2019).
Cited by
23 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献