Author:
Al-Qaoud Khaled M.,Obeidat Yusra M.,Al-Omari Tareq,Okour Mohammad,Al-Omari Mariam M.,Ahmad Mohammad I.,Alshadfan Raed,Rawashdeh AbdelMonem M.
Abstract
AbstractThis paper introduces a novel approach for detecting the SARS-CoV-2 recombinant spike protein combining a label free electrochemical impedimetric immunosensor with the use of purified chicken IgY antibodies. The sensor employs three electrodes and is functionalized with an anti-S IgY antibody, ELISA and immunoblot assays confirmed the positive response of chicken immunized with SARS-CoV2 S antigen. The developed immunosensor is effective in detecting SARS-CoV-2 in nasopharyngeal clinical samples from suspected cases. The key advantage of this biosensor is its remarkable sensitivity, and its capability of detecting very low concentrations of the target analyte, with a detection limit of 5.65 pg/mL. This attribute makes it highly suitable for practical point-of-care (POC) applications, particularly in low analyte count clinical scenarios, without requiring amplification. Furthermore, the biosensor has a wide dynamic range of detection, spanning from 11.56 to 740 ng/mL, which makes it applicable for sample analysis in a typical clinical setting.
Funder
the Scientific Research and Innovation Fund
Publisher
Springer Science and Business Media LLC
Cited by
5 articles.
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