Use of Chimeric Enzymes and Site-Directed Mutagenesis for Identification of Three Key Residues Responsible for Differences in Steroid Hydroxylation between Canine Cytochromes P-450 3A12 and 3A26-Reference-Cited by-同舟云学术

Use of Chimeric Enzymes and Site-Directed Mutagenesis for Identification of Three Key Residues Responsible for Differences in Steroid Hydroxylation between Canine Cytochromes P-450 3A12 and 3A26

Published:1999-02-01 Issue:2 Volume:55 Page:241-247
ISSN:0026-895X
Container-title:Molecular Pharmacology
language:en
Short-container-title:Mol Pharmacol

Author:

Fraser David J.,He You Qun,Harlow Greg R.,Halpert James R.

Publisher

American Society for Pharmacology & Experimental Therapeutics (ASPET)

Subject

Pharmacology,Molecular Medicine

Reference23 articles.

1. Sequence requirements for cytochrome P450 IIB1 catalytic activity: Alteration of the stereospecificity and regioselectivity of steroid hydroxylation by a simultaneous change of two hydrophobic amino acid residues to phenylalanine.;Aoyama;J Biol Chem,1989

2. Expression and enzymatic activity of recombinant cytochrome P450 17 alpha-hydroxylase in Escherichia coli.

3. Escherichia coli expression and substrate specificities of canine cytochrome P450 3A12 and rabbit cytochrome P450 3A6.;Born;J Pharmacol Expt Ther,1996

4. cDNA and deduced amino acid sequences of a dog liver cytochrome P-450 of the IIIA gene subfamily.;Ciaccio;Biochim Biophys Acta,1991

5. Analysis of Four Residues within Substrate Recognition Site 4 of Human Cytochrome P450 3A4: Role in Steroid Hydroxylase Activity and α-Naphthoflavone Stimulation

Cited by 16 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. An Expanded, Unified Substrate Recognition Site Map for Mammalian Cytochrome P450s: Analysis of Molecular Interactions Between 15 Mammalian CYP450 Isoforms and 868 Substrates;Current Drug Metabolism;2011-09-01

2. High-throughput enzymology and combinatorial mutagenesis for mining cytochrome P450 functions;Expert Opinion on Drug Metabolism & Toxicology;2008-06

3. Mutation of a Single Residue (K262R) in P450 2B6 Leads to Loss of Mechanism-Based Inactivation by Phencyclidine;Drug Metabolism and Disposition;2007-04-25

4. Re-engineering cytochrome P450 2B11dH for enhanced metabolism of several substrates including the anti-cancer prodrugs cyclophosphamide and ifosfamide;Archives of Biochemistry and Biophysics;2007-02

5. Analysis of the GTPase Activity and Active Sites of the NG Domains of FtsY and Ffh from Streptomyces coelicolor;Acta Biochimica et Biophysica Sinica;2006-07