evSeq: Cost-Effective Amplicon Sequencing of Every Variant in a Protein Library-Reference-Cited by-同舟云学术

evSeq: Cost-Effective Amplicon Sequencing of Every Variant in a Protein Library

Published:2022-02-17 Issue:3 Volume:11 Page:1313-1324
ISSN:2161-5063
Container-title:ACS Synthetic Biology
language:en
Short-container-title:ACS Synth. Biol.

Author:

Wittmann Bruce J.¹^ORCID,Johnston Kadina E.¹^ORCID,Almhjell Patrick J.²^ORCID,Arnold Frances H.¹²^ORCID

Affiliation:

1. Division of Biology and Biological Engineering, California Institute of Technology, MC 210-41, 1200 E. California Boulevard, Pasadena, California 91125, United States

2. Division of Chemistry and Chemical Engineering, California Institute of Technology, MC 210-41, 1200 E. California Boulevard, Pasadena, California 91125, United States

Funder

Basic Energy Sciences

Amgen

Division of Chemical, Bioengineering, Environmental, and Transport Systems

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

Link

https://pubs.acs.org/doi/pdf/10.1021/acssynbio.1c00592

Reference46 articles.

1. BCC Research Staff Global Markets for Enzymes in Industrial Applications; BCC Research LLC, 2018. https://www.bccresearch.com/market-research/biotechnology/global-markets-for-enzymes-in-industrial-applications.html.

2. Pervasive degeneracy and epistasis in a protein-protein interface

3. Adaptation in protein fitness landscapes is facilitated by indirect paths

4. Global mapping of the energetic and allosteric landscapes of protein binding domains

5. Machine-learning-guided directed evolution for protein engineering

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