Development of a Freeze-Dried CRISPR-Cas12 Sensor for Detecting Wolbachia in the Secondary Science Classroom

Author:

Rybnicky Grant A.123ORCID,Dixon Radeen A.4,Kuhn Robert M.45,Karim Ashty S.126,Jewett Michael C.12678ORCID

Affiliation:

1. Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois 60208, United States

2. Center for Synthetic Biology, Northwestern University, Evanston, Illinois 60208, United States

3. Interdisciplinary Biological Sciences Graduate Program, Northwestern University, Evanston, Illinois 60208, United States

4. Centennial High School, 9310 Scott Rd, Roswell, Georgia 30076, United States

5. Innovation Academy Fulton County Schools STEM Magnet High School, 125 Milton Avenue, Alpharetta, Georgia 30009, United States

6. Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States

7. Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois 60611, United States

8. Simpson Querrey Institute, Northwestern University, Chicago, Illinois 60611, United States

Funder

Army Research Office

Air Force Office of Scientific Research

Camille and Henry Dreyfus Foundation

David and Lucile Packard Foundation

Office of Energy Efficiency and Renewable Energy

U.S. Department of Energy

Defense Threat Reduction Agency

National Science Foundation

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

Reference31 articles.

1. Synthetic Biology Market by Tools (Oligonucleotides, Enzymes, Synthetic Cells), Technology (Gene Synthesis, Genome Engineering), Application(Tissue Regeneration, Biofuel, Consumer Care, Food & Agriculture, Environmental) and Region - Global Forecast to 20, 2021.

2. BioBits™ Bright: A fluorescent synthetic biology education kit

3. BioBits™ Explorer: A modular synthetic biology education kit

4. BioBits Health: Classroom Activities Exploring Engineering, Biology, and Human Health with Fluorescent Readouts

5. An educational module to explore CRISPR technologies with a cell-free transcription-translation system

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