BioBits™ Bright: A fluorescent synthetic biology education kit

Author:

Stark Jessica C.123ORCID,Huang Ally456ORCID,Nguyen Peter Q.67ORCID,Dubner Rachel S.8ORCID,Hsu Karen J.9,Ferrante Thomas C.6,Anderson Mary10,Kanapskyte Ada11ORCID,Mucha Quinn10,Packett Jessica S.11,Patel Palak10,Patel Richa10,Qaq Deema10,Zondor Tyler10,Burke Julie12ORCID,Martinez Thomas10,Miller-Berry Ashlee13,Puppala Aparna14,Reichert Kara15ORCID,Schmid Miriam16,Brand Lance17ORCID,Hill Lander R.18ORCID,Chellaswamy Jemima F.19ORCID,Faheem Nuhie20ORCID,Fetherling Suzanne21,Gong Elissa22,Gonzalzles Eddie Marie23,Granito Teresa13,Koritsaris Jenna13,Nguyen Binh24,Ottman Sujud25ORCID,Palffy Christina26,Patel Angela27,Skweres Sheila13,Slaton Adriane13ORCID,Woods TaRhonda13,Donghia Nina6ORCID,Pardee Keith28ORCID,Collins James J.4562930ORCID,Jewett Michael C.1233132ORCID

Affiliation:

1. Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Technological Institute E136, Evanston, IL 60208–3120, USA.

2. Chemistry of Life Processes Institute, Northwestern University, 2170 Campus Drive, Evanston, IL 60208–3120, USA.

3. Center for Synthetic Biology, Northwestern University, 2145 Sheridan Road, Technological Institute E136, Evanston, IL 60208–3120, USA.

4. Department of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.

5. Institute for Medical Engineering and Science, MIT, Cambridge, MA 02139, USA.

6. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.

7. School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.

8. Department of Biological Sciences, Northwestern University, 2205 Tech Drive, Hogan Hall 2144, Evanston, IL 60208, USA.

9. Department of Mechanical Engineering, Northwestern University, 2145 Sheridan Road, Technological Institute B224, Evanston, IL 60208–3120, USA.

10. Glenbard East High School, 1014 South Main Street, Lombard, IL 60148–3938, USA.

11. Amos Alonzo Stagg High School, 8015 West 111th Street, Palos Hills, IL 60465–2203, USA.

12. Grover Cleveland Elementary School, 3121 West Byron Street, Chicago, IL 60618–3403, USA.

13. Evanston Township High School, 1600 Dodge Avenue, Evanston, IL 60201–3449, USA.

14. Glenbrook South High School, 4000 West Lake Avenue, Glenview, IL 60026–1239, USA.

15. Jones College Prep High School, 700 South State Street, Chicago, IL 60605–2109, USA.

16. Gwendolyn Brooks College Preparatory Academy, 250 East 111th Street, Chicago, IL 60628–4324, USA.

17. Delta High School, 3400 East SR 28, Muncie, IN 47303, USA.

18. ASPIRA Business and Finance High School, 2989 North Milwaukee Avenue, Chicago, IL 60618–7347, USA.

19. Aptakisic-Tripp School District 102, 850 Highland Grove Drive, Buffalo Grove, IL 60089, USA.

20. Oak Lawn Hometown Middle School, 5345 West 99th Street, Oak Lawn, IL 60453–3815, USA.

21. Hoffman Estates High School, 1100 West Higgins Road, Hoffman Estates, IL 60169–4050, USA.

22. Vernon Hills High School, 145 Lakeview Parkway, Vernon Hills, IL 60061–1566, USA.

23. Morgan Park High School, 1744 West Pryor Avenue, Chicago, IL 60643–3497, USA.

24. Northside College Prep High School, 5501 North Kedzie Avenue, Chicago, IL 60625–3923, USA.

25. Aqsa School, 7361 West 92nd Street, Bridgeview, IL 60455–2133, USA.

26. Adlai E. Stevenson High School, 1 Stevenson Drive, Lincolnshire, IL 60069–2824, USA.

27. Lyons Township High School, 100 South Brainard Avenue, La Grange, IL 60525–2101, USA.

28. Leslie Dan Faculty of Pharmacy, University of Toronto, 144 College Street, Toronto, Ontario M5S 3M2, Canada.

29. Synthetic Biology Center, MIT, Cambridge, MA 02139, USA.

30. Harvard-MIT Program in Health Sciences and Technology, Cambridge, MA 02139, USA.

31. Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 676 North Saint Clair Street, Suite 1200, Chicago, IL 60611–3068, USA.

32. Simpson Querrey Institute, Northwestern University, 303 East Superior Street, Suite 11-131, Chicago, IL 60611–2875, USA.

Abstract

We report portable, user-friendly reagents and equipment for visual, hands-on biology activities with supporting curriculum.

Funder

National Science Foundation

David and Lucile Packard Foundation

Air Force Office of Scientific Research

Army Research Office

Wyss institute

Paul G. Allen Frontiers Group

Defense Threat Reduction Agency

Camille Dreyfus Teacher-Scholar Program

Air Force Research Laboratory Center of Excellence

Natural Sciences and Engineering Council of Canada

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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