Abstract
Mutants of
Escherichia coli
impaired in fructose 1-phosphate kinase activity (Fpk) accumulate fructose 1-phosphate from fructose, which arrests their growth. Phenotypic revertants to fructose tolerance have lost either the histidine-containing protein (HPr) or enzyme I of the phosphoenolpyruvate phosphotransferase system (
ctr
), and consequently utilize neither fructose nor glucose, or an enzyme II specific for the uptake of fructose and its concomitant phosphorylation to fructose 1-phosphate (PtsF). However, PtsF
—
-mutants can still grow on high concentrations ( > 2 mM) of fructose, and take up this sugar via a low-affinity enzyme II designated PtsX that effects its uptake and phosphorylation to fructose 6-phosphate. Mutants of the Hfr-strain KL16 were isolated that lacked PtsF, or PtsF and PtsX, activities. The consequence to, and rôle of these functions in, the uptake and metabolism of fructose are described.
Reference30 articles.
1. The anaplerotic fixation of carbon dioxide by
Escherichia coli
2. B ourd G. I. B olshakova T. N . S aprykina T. P . K Iy utcheva V. V. & G ershanovich V. N . 1971 Decrease of the ra te of R N A and protein synthesis in th e therm osensitive E . coli K 1 2 m u ta n t defective in th e R o sem an 's phosphotransferase system . M olek. 3 384-389.
3. A proposal for a uniform nom enclature in b acterial genetics;Clark A. J .;Genetics,1966
4. Pathway of fructose utilization byEscherichia coli
5. Role of fructose-1,6-diphosphatase in fructose utilization byEscherichia coli
Cited by
58 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献