Development of a rapid detection method for molecular beacons LAMP for Vibrio parahaemolyticus in food safety

Abstract

A bacteria called Vibrio parahaemolyticus can result in food poisoning; therefore, Finding Vibrio parahaemolyticus in food quickly is essential to reduce food poisoning. A MB-LAMP fast detection technique was created in this work to identify Vibrio parahaemolyticus DNA in food products with 102 CFU/mL of sensitivity and absence of cross-reactivity with four additional bacteria. For 20 hypothetical instances, the MB-LAMP system was consistent with the commercial Vibrio parahaemolyticus qPCR kit, and the former reduced the experimental time by about 35 min compared with the latter.