Usage of the tissue culture method for the development of new forms of Fagopyrum esculentum Moench

Abstract

The article presents results of usage of the tissue culture method on buckwheat (Fagopyrum esculentum Moench) together with the selective factor increased concentrations of heavy metal ions in the nutrient medium. On the basis of Izumrud (Russia), Kitavase (Japan), hybrid Izumrud x Kitavase there were developed regenerant lines tolerant to copper and zinc. Negative action effect of copper ions was observed from the second generation in the second posterity of the plants-regenerants, developed from the callus, in the form of the chlorophyll-free mutants. Positive effect of the heavy metals was observed on increasing of rutin content of the plants-regenerants in vitro. Selective evaluation in the field conditions of the samples tolerant to heavy metal let to choose from them the most adaptive to monsoon conditions in Primorskykrai, which combine valuable traits of big grain (weight of 1000 grains 36-38 g), high seed productivity (1,9-3,2 g) and high rutin content in aboveground mass of plants (2,8-4,3 %).