Caterpillar-Induced Volatile Emissions in Cotton: The Relative Importance of Damage and Insect-Derived Factors

Abstract

In response to herbivore attack, plants release large amounts of volatiles that can serve as attractants for the natural enemies of the attacking herbivores. Such responses are typically triggered by damage- and insect-associated factors. Cotton plants are somewhat peculiar because they release specific blends of volatiles in two waves in response to caterpillar attack. They first emit constitutively stored volatile compounds, and after about 24 h a second wave that includes various de novo synthesized compounds. The relative importance of damage-associated and insect associated-factors in this induction of cotton volatile emissions is not yet fully clear. We evaluated how cotton plants respond to mechanical damage and to the application of the oral secretion from the generalist lepidopteran pest Spodoptera exigua, by measuring the local and systemic emissions of volatile compounds from their leaves. Our results confirm that cotton plants respond to damage-associated molecular patterns (DAMPs) as well as to herbivore-associated molecular patterns (HAMPs) present in the caterpillars’ oral secretion. Interestingly, a stronger response was observed for cotton plants that were treated with oral secretion from cotton-fed caterpillars than those fed on maize. We tested the possibility that volicitin, a common fatty acid-derived elicitor in caterpillar regurgitant plays a role in this difference. Volicitin and volicitin-like compounds were detected in equal amounts in the oral secretion of S. exigua fed on either cotton or maize leaves. We conclude that other elicitors must be involved. The identification of these eliciting cues is expected to contribute to the development of novel strategies to enhance the resistance of cotton plants to insect pests.