Classification of Hen Eggs by HPLC-UV Fingerprinting and Chemometric Methods

Author:

Campmajó ,Cayero ,Saurina ,Núñez

Abstract

Hen eggs are classified into four groups according to their production method: Organic, free-range, barn, or caged. It is known that a fraudulent practice is the misrepresentation of a high-quality egg with a lower one. In this work, high-performance liquid chromatography with ultraviolet detection (HPLC-UV) fingerprints were proposed as a source of potential chemical descriptors to achieve the classification of hen eggs according to their labelled type. A reversed-phase separation was optimized to obtain discriminant enough chromatographic fingerprints, which were subsequently processed by means of principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). Particular trends were observed for organic and caged hen eggs by PCA and, as expected, these groupings were improved by PLS-DA. The applicability of the method to distinguish egg manufacturer and size was also studied by PLS-DA, observing variations in the HPLC-UV fingerprints in both cases. Moreover, the classification of higher class eggs, in front of any other with one lower, and hence cheaper, was studied by building paired PLS-DA models, reaching a classification rate of at least 82.6% (100% for organic vs. non-organic hen eggs) and demonstrating the suitability of the proposed method.

Funder

Generalitat de Catalunya

Publisher

MDPI AG

Subject

Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science

Reference23 articles.

1. Fatty Acid Composition and Egg Components of Specialty Eggs

2. Fatty acid and carotenoid composition of egg yolk as an effect of microalgae addition to feed formula for laying hens

3. Agriculture and Rural Developmenthttps://www.ec.europa.eu/agriculture/eggs_en

4. Council Directive 1999/74/EC of 19 July 1999 laying down minimum standards for the protection of laying hens;Off. J. Eur. Communities,1999

5. Commission Directive 2002/4/EC of 30 January 2002 on the registration of establishments keeping laying hens, covered by Council Directive 1999/74/EC;Off. J. Eur. Communities,2002

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