Bioconversion of a Lignocellulosic Hydrolysate to Single Cell Oil for Biofuel Production in a Cost-Efficient Fermentation Process

Author:

Rerop Zora S.1ORCID,Stellner Nikolaus I.1ORCID,Graban Petra1,Haack Martina1,Mehlmer Norbert1ORCID,Masri Mahmoud1ORCID,Brück Thomas B.1ORCID

Affiliation:

1. Werner Siemens-Chair of Synthetic Biotechnology, School of Natural Sciences, Technical University of Munich, Lichtenbergstraße 4, 85748 Garching bei München, Germany

Abstract

Cutaneotrichosporon oleaginosus is a highly efficient single cell oil producer, which in addition to hexoses and pentoses can metabolize organic acids. In this study, fed-batch cultivation with consumption-based acetic acid feeding was further developed to integrate the transformation of an industrial paper mill lignocellulosic hydrolysate (LCH) into yeast oil. Employing pentose-rich LCH as a carbon source instead of glucose significantly improved both biomass formation and lipid titer, reaching 55.73 ± 5.20 g/L and 42.1 ± 1.7 g/L (75.5% lipid per biomass), respectively. This hybrid approach of using acetic acid and LCH in one process was further optimized to increase the share of bioavailable carbon from LCH using a combination of consumption-based and continuous feeding. Finally, the techno-economic analysis revealed a 26% cost reduction when using LCH instead of commercial glucose. In summary, we developed a process leading to a holistic approach to valorizing a pentose-rich industrial waste by converting it into oleochemicals.

Funder

The German Federal Ministry of Education and Research

European Union’s Horizon Europe research and innovation program

Werner Siemens Foundation

Publisher

MDPI AG

Subject

Plant Science,Biochemistry, Genetics and Molecular Biology (miscellaneous),Food Science

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