Structural Characterization and Immunological Activity of Polysaccharide Degradation Products from Phlebopus portentosus

Author:

Yu Dan12,Cai Xiaoming12,Wang Shuo13,Li Yi1ORCID,Du Yuguang24,Wang Zhuo A.24ORCID,Jiao Siming24,Yang Zhenquan1

Affiliation:

1. School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China

2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

3. Jiangsu Alphay Bio-Technology Co., Ltd., Nantong 226010, China

4. Key Laboratory of Biopharmaceutical Preparation and Delivery, Chinese Academy of Sciences, Beijing 100190, China

Abstract

Phlebopus portentosus is an edible and medicinal mushroom with a delicious taste and high nutritional value. The oligosaccharides derived from P. portentosus may be the material basis for its biological activity. The degradation of polysaccharide and the maintenance of its activity after degradation are key steps in related research. This study applied an acid degradation method to prepare P. portentosus refined polysaccharide (PPRP) with a smaller molecular weight, and the optimal hydrolysis conditions determined were a temperature of 80 °C, an acid concentration of 2 mol/L, and a hydrolysis time of 2 h. The polysaccharide structure and immune activity were then further investigated. The results showed that the PPRP comprised two fractions with approximate weights of 61,600 Da and 5500 Da. The monosaccharide composition of PPRP was mannose, rhamnose, glucose, and galactose, with a molar ratio of 1.00: 22.24: 2.93: 1.03. The major functional groups included O-H, C-H, C-O, and C-O-C. The glycosidic bond types were mainly α- and β-glycosidic bonds. Cell experiments demonstrated that PPRP could significantly increase the proliferation of macrophages and enhance the cytotoxicity of NK cells. Moreover, PPRP also significantly promoted the proliferation of B lymphocytes and T lymphocytes, especially at a concentration of 200 μg/mL. This study furnishes scientific evidence underlining the significant potential of PPRP in immune activity, thereby serving as a material basis and scientific bedrock for further investigations into the mechanism of P. portentosus oligosaccharide activity.

Funder

National Key R&D Program of China

Investigation of Wild Germplasm Resources of Agricultural Microorganisms (Edible Fungi) in Jiangsu Province

Publisher

MDPI AG

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