Assignment of Focus Position with Convolutional Neural Networks in Adaptive Lens Based Axial Scanning for Confocal Microscopy

Abstract

Adaptive lenses offer axial scanning without mechanical translation and thus are promising to replace mechanical-movement-based axial scanning in microscopy. The scan is accomplished by sweeping the applied voltage. However, the relation between the applied voltage and the resulting axial focus position is not unambiguous. Adaptive lenses suffer from hysteresis effects, and their behaviour depends on environmental conditions. This is especially a hurdle when complex adaptive lenses are used that offer additional functionalities and are controlled with more degrees of freedom. In such case, a common approach is to iterate the voltage and monitor the adaptive lens. Here, we introduce an alternative approach which provides a single shot estimation of the current axial focus position by a convolutional neural network. We use the experimental data of our custom confocal microscope for training and validation. This leads to fast scanning without photo bleaching of the sample and opens the door to automatized and aberration-free smart microscopy. Applications in different types of laser-scanning microscopes are possible. However, maybe the training procedure of the neural network must be adapted for some use cases.