Leaf Carbohydrate Metabolism Variation Caused by Late Planting in Rapeseed (Brassica napus L.) at Reproductive Stage

Abstract

Delayed planting date of rapeseed is an important factor affecting seed yield. However, regulation of the leaf carbohydrate metabolism in rapeseed by a late planting date at the reproductive stage is scarcely investigated. A two-year field experiment was conducted to assess the effect of planting dates, including early (15 September), optimal (1 October), late (15 October), and very late (30 October), on leaf growth and carbohydrate biosynthetic and catabolic metabolism at the reproductive stage. The results showed that leaf dry matter decreased linearly on average from 7.48 to 0.62 g plant−1 with an early planting date, whereas it increased at first and peaked at 14 days after anthesis (DAA) with other planting dates. Leaf dry matter was the lowest at the very late planting date during the reproductive stage. For leaf chlorophyll content, rapeseed planted at an optimal date maximized at 14 DAA with an average content of 1.51 mg g−1 fresh weight, whereas it kept high and stable at a very late planting date after 28 DAA. For the carbohydrate catabolic system, acid and neutral invertase (AI and NI, respectively) showed higher activity before 14 DAA, whereas both sucrose synthase (SS) and starch phosphorylase (SP) showed higher activity after 14 DAA. For the carbohydrate biosynthetic system, the activity of sucrose phosphate synthase (SPS) was the highest at the late planting date after 14 DAA, whereas it was at the lowest at the very late planting date. However, the activity of ADP-glucose pyrophosphorylase (AGPase) at the late and very late planting dates was significantly higher than that of the early and optimal plant dates after 21 DAA, which is in accordance with the leaf total soluble sugar content, suggesting that leaf carbohydrate metabolism is governed by a biosynthetic system. The current study provides new insights on leaf carbohydrate metabolism regulation by late planting in rapeseed at the reproductive stage.