Abstract
The biotransformation of the aromatic amino acids phenylalanine, tyrosine and tryptophan originates a number of bioactive compounds. Yeasts are the most used microorganisms for the transformation of (L)-phenylalanine into the flavour phenylethanol. Here, we reported a study on the biotransformation of the proteogenic aminoacids phenylalanine, tyrosine and tryptophan by yeast strains belonging to Yarrowia genus. We found that the latter microorganisms, in high aerobic conditions, metabolise the aromatic amino acids (L)-phenylalanine and (L)-tyrosine with the almost exclusive formation of phenylacetic acid and 4-hydroxyphenylacetic acid, respectively. Differently, the biotransformation of (L)-tryptophan with Y. lipolytica, gave anthranilic acid as the main product. As stated by the European and USA legislations concerning natural flavour production, phenylacetic acid obtained by microbial conversion of phenylalanine of natural origin can be commercialised as a natural flavour. Accordingly, our findings were exploited in a new process, based on the Yarrowia strains-mediated biotransformation of natural (L)-phenylalanine, that allows the large-scale preparation of the high-value, natural flavour, phenylacetic acid.
Subject
Physical and Theoretical Chemistry,Catalysis,General Environmental Science
Reference56 articles.
1. The Ehrlich pathway for fusel alcohol production: A century of research on Saccharomyces cerevisiae metabolism;Hazelwood;Appl. Environ. Microbiol.,2008
2. Current status and perspectives of 2-phenylethanol production through biological processes;Qian;Crit. Rev. Biotechnol.,2019
3. Farbood, M.I., Blocker, R.W., McLean, L.B., and Scharpf, L.G. (1995). Fermentation Process for Preparing Phenylacetic Acid Using Phenylalanine as A Starting Material. (US5420022), U.S. Patent.
4. Matsumoto, M., Narita, M., Yamauchi, S., and Nakajima, T. (1984). Production of Phenylacetic Acid. (JPS59232095(A)), Japanese Patent.
5. Gluconobacter oxydans used to production of natural aroma—2-Phenylacetic acid in immobilized system (lentikats form);Rosenberg;New Biotechnol.,2014