Iron Deficiency Modulates Secondary Metabolites Biosynthesis and Antioxidant Potential in Sulla carnosa L. Primed with Salicylic Acid

Abstract

Iron (Fe) is a vital nutrient for the development of many plants. Therefore, enhancing plant performance and production in relation to Fe deficiency is becoming a serious challenge. In this work, we intended to survey the effect of seed pretreatment with salicylic acid (SA) on oxidative status, phenolic composition, and related antioxidant activities in two varieties of Sulla carnosa (Sidi Khlif: SK and Kalbia: KA) treated with different Fe concentrations. In unprimed plants, the levels of phenolic compounds were very distinguishable among the two varieties, being superior in KA compared to SK. Interestingly, priming KA seeds with SA under control conditions or deficient conditions (D+SA treatment) caused a decreasing tendency in the contents of total phenolic compounds (TPC) and total flavonoids (TFC), whereas an opposite behavior was observed in SK. Moreover, an improvement of hydroxycinnamic and flavonoid groups, in addition to antioxidant activities (TAC, DPPH●, β-carotene and FRAP), was markedly found in SK primed with SA. According to these findings, SA seed pretreatment had a beneficial effect on the metabolic performance of this species under different Fe supply, regardless of whether the intensity of improvement was related to Fe concentration applied, variety, as well as plant organ. The results suggest that SA can account for the effective modulation of the secondary metabolites metabolism in S. carnosa plants to deal with the detrimental impacts of Fe deficiency.