Novel Amplicon-Based Sequencing Approach to West Nile Virus-Reference-Cited by-同舟云学术

Novel Amplicon-Based Sequencing Approach to West Nile Virus

Published:2023-05-27 Issue:6 Volume:15 Page:1261
ISSN:1999-4915
Container-title:Viruses
language:en
Short-container-title:Viruses

Author:

Diagne Moussa Moïse¹^ORCID,Ndione Marie Henriette Dior¹^ORCID,Mencattelli Giulia²³⁴^ORCID,Diallo Amadou⁵^ORCID,Ndiaye El hadji⁶^ORCID,Di Domenico Marco²^ORCID,Diallo Diawo⁶^ORCID,Kane Mouhamed¹,Curini Valentina²^ORCID,Top Ndeye Marieme⁵,Marcacci Maurilia²^ORCID,Mbanne Maïmouna¹,Ancora Massimo²^ORCID,Secondini Barbara²,Di Lollo Valeria²,Teodori Liana²,Leone Alessandra²,Puglia Ilaria²^ORCID,Gaye Alioune⁶^ORCID,Sall Amadou Alpha¹,Loucoubar Cheikh⁵^ORCID,Rosà Roberto³^ORCID,Diallo Mawlouth⁶,Monaco Federica²^ORCID,Faye Ousmane¹,Cammà Cesare²^ORCID,Rizzoli Annapaola⁴^ORCID,Savini Giovanni²^ORCID,Faye Oumar¹^ORCID

Affiliation:

1. Virology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal

2. Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy

3. Centre Agriculture Food Environment, University of Trento, 38010 San Michele all’Adige, Italy

4. Research and Innovation Centre, Fondazione Edmund Mach, 38010 San Michele all’Adige, Italy

5. Epidemiology, Clinical Research and Data Science Department, Institut Pasteur de Dakar, Dakar BP220, Senegal

6. Medical Zoology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal

Abstract

West Nile virus is a re-emerging arbovirus whose impact on public health is increasingly important as more and more epidemics and epizootics occur, particularly in America and Europe, with evidence of active circulation in Africa. Because birds constitute the main reservoirs, migratory movements allow the diffusion of various lineages in the world. It is therefore crucial to properly control the dispersion of these lineages, especially because some have a greater health impact on public health than others. This work describes the development and validation of a novel whole-genome amplicon-based sequencing approach to West Nile virus. This study was carried out on different strains from lineage 1 and 2 from Senegal and Italy. The presented protocol/approach showed good coverage using samples derived from several vertebrate hosts and may be valuable for West Nile genomic surveillance.

Funder

Africa Pathogen Genomics Initiative (Africa PGI) through the Bill & Melinda Gates Foundation

Institut Pasteur de Dakar internal funds

international PhD initiative including Fondazione Edmund Mach, University of Trento, and Istituto Zooprofilattico of Teramo

Publisher

MDPI AG

Subject

Virology,Infectious Diseases

Link

https://www.mdpi.com/1999-4915/15/6/1261/pdf

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