Characterization and Genome Analysis of Cladobotryum mycophilum, the Causal Agent of Cobweb Disease of Morchella sextelata in China

Author:

Liu Zhenghui12,Cong Yunlong3,Sossah Frederick Leo24ORCID,Lu Yongzhong5,Kang Jichuan1,Li Yu2

Affiliation:

1. Engineering and Research Center for Southwest Bio-pharmaceutical Resources of National Education Ministry, Guizhou University, Guiyang 550025, China

2. Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, China

3. Research Institute of Science and Technology, Guizhou University, Guiyang 550025, China

4. Council for Scientific and Industrial Research (CSIR), Oil Palm Research Institute, Coconut Research Programme, Sekondi P.O. Box 245, Ghana

5. School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, China

Abstract

Cobweb disease is a fungal disease that can cause serious damage to edible mushrooms worldwide. To investigate cobweb disease in Morchella sextelata in Guizhou Province, China, we isolated and purified the pathogen responsible for the disease. Through morphological and molecular identification and pathogenicity testing on infected M. sextelata, we identified Cladobotryum mycophilum as the cause of cobweb disease in this region. This is the first known occurrence of this pathogen causing cobweb disease in M. sextelata anywhere in the world. We then obtained the genome of C. mycophilum BJWN07 using the HiFi sequencing platform, resulting in a high-quality genome assembly with a size of 38.56 Mb, 10 contigs, and a GC content of 47.84%. We annotated 8428 protein-coding genes in the genome, including many secreted proteins, host interaction-related genes, and carbohydrate-active enzymes (CAZymes) related to the pathogenesis of the disease. Our findings shed new light on the pathogenesis of C. mycophilum and provide a theoretical basis for developing potential prevention and control strategies for cobweb disease.

Funder

uizhou Provincial Department of Education Young Science and Technology Talent Growth

Guizhou University Natural Science Special (Special Post) Scientific Research Fund Project

Strategic Pilot Science and Technology Project of Chinese Academy of Sciences

The National Key Research and Development Program of China

The Natural Science Foundation of Guizhou Province

Publisher

MDPI AG

Subject

Plant Science,Ecology, Evolution, Behavior and Systematics,Microbiology (medical)

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