Antibacterial Activity and Mechanism of Polygonum orientale L. Essential Oil against Pectobacterium carotovorum subsp. carotovorum

Abstract

Infected by Pectobacterium carotovorum subsp. carotovorum (Pcc), the quality of Chinese cabbage could severely decline. Using chemical bactericides to control Pcc could cause food safety problems. Thus, we investigated the optimum extraction conditions, antibacterial activity, chemical compounds and antibacterial mechanism of Polygonum orientale L. essential oil (POEO) against Pcc in order to search a new way to control Pcc. The optimum extraction conditions of POEO (soaking time 2.6 h, extraction time 7.7 h and ratio of liquid to solid 10.3 mL/g) were optimized by response surface methodology. The minimum inhibitory concentration (MIC) of POEO against Pcc was 0.625 mg/mL. The control efficiency of protective activity of POEO against Pcc was 74.67~92.67%, and its curative activity was 76.00~93.00%. Then, 29 compounds were obtained by GC-MS; the prime compounds of POEO were phytol, phytone, n-pentacosane, 1-octen-3-ol and β-ionone. It was verified that, compared with control samples, POEO destroyed cell morphology. It increased surface potential, increased hydrophobicity, damaged cell walls, destroyed the integrity and permeability of cell membrane, reduced membrane potential (MP), and changed membrane protein conformation. It inhibited the activities of pyruvate kinase (PK), succinate dehydrogenase (SDH) and adenosine triphosphatase (ATPase). Briefly, the results of this study demonstrate that POEO showed effective inhibitory activity against Pcc, thus POEO could have potential application in controlling Pcc.