Near Infrared Spectroscopic Evaluation of Starch Properties of Diverse Sorghum Populations

Abstract

Starch, mainly composed of amylose and amylopectin, is the major nutrient in grain sorghum. Amylose and amylopectin composition affects the starch properties of sorghum flour which in turn determine the suitability of sorghum grains for various end uses. Partial least squares regression models on near infrared (NIR) spectra were developed to estimate starch and amylose contents in intact grain sorghum samples. Sorghum starch calibration model with a coefficient of determination (R2) = 0.87, root mean square error of cross validation (RMSECV) = 1.57% and slope = 0.89 predicted the starch content of validation set with R2 = 0.76, root mean square error of prediction (RMSEP) = 2.13%, slope = 0.93 and bias = 0.20%. Amylose calibration model with R2 = 0.84, RMSECV = 2.96% and slope = 0.86 predicted the amylose content in validation samples with R2 = 0.76, RMSEP = 2.60%, slope = 0.98 and bias = −0.44%. Final starch and amylose cross validated calibration models were constructed combining respective calibration and validation sets and used to predict starch and amylose contents in 1337 grain samples from two diverse sorghum populations. Protein and moisture contents of the samples were determined using previously tested NIR spectroscopy models. The distribution of starch and protein contents in the samples of low amylose (<5%) and normal amylose (>15%) and the overall relationship between starch and protein contents of the sorghum populations were investigated. Percent starch and protein were negatively correlated, low amylose lines tended to have lower starch and higher protein contents than lines with high amylose. The results showed that NIR spectroscopy of whole grain can be used as a high throughput pre-screening method to identify sorghum germplasm with specific starch quality traits to develop hybrids for various end uses.