Affiliation:
1. Procesos de Polimerizacion Centro de Investigación en Química Aplicada Saltillo Coahuila de Zaragoza Mexico
2. Facultad de Ciencias Químicas Universidad Autónoma de Coahuila Saltillo Coahuila de Zaragoza Mexico
3. Investigadoras e Investigadores por México CONAHCyT Av. Insurgentes sur Mexico City 03940 Mexico
Abstract
AbstractIntroductionPectin‐oligosaccharides (POS) serve diverse purposes as a food ingredient, antimicrobial and biostimulant in plants, and their functionality is linked to the degree of esterification. Grape and broccoli wastes emerge as environmentally friendly alternatives to obtaining pectin, serving as a sustainable source to producing POS. For example, microwaves have proven to be an effective and sustainable method to extract polysaccharides from plant matrices.ObjectiveThis work aims to use grape and broccoli wastes as alternative sources for obtaining pectin by microwave‐assisted extraction and biotransformation into POS, which possess biological properties.Material and MethodsThe extraction conditions were identified at a power of 400 W, 300 s for the extraction of pectin from grape pomace and broccoli waste. Biotransformation of pectins into POS, using commercial enzyme preparations (Viscozyme L and Pectinase). Characterisation was carried out by Fourier‐transform infrared spectroscopy, thermogravimetric analysis, and scanning electron microscopy.ResultsPhysicochemical analysis indicated grape pomace and broccoli waste pectins had galacturonic acid content of 63.81 ± 1.67 and 40.83 ± 2.85 mg 100 mg−1, low degree of esterification of 34.89% and 16.22%, respectively. Biotransformation of pectins into POS resulted in a 20% hydrolysis rate. The main enzymatic activity was polygalacturonase for the degradation of the main structure of the pectin.ConclusionProduction of POS from agro‐industrial wastes by emerging technologies, such as the combined use of microwave‐assisted extraction and enzymatic processes, represents an alternative method for the generation of bioactive compounds with distinctive properties suitable for different applications of interest.