Generating CRISPR‐Cas9‐Mediated Null Mutations and Screening Targeting Efficiency in Human Pluripotent Stem Cells-Reference-Cited by-同舟云学术

Generating CRISPR‐Cas9‐Mediated Null Mutations and Screening Targeting Efficiency in Human Pluripotent Stem Cells

Published:2021-08 Issue:8 Volume:1 Page:
ISSN:2691-1299
Container-title:Current Protocols
language:en
Short-container-title:Current Protocols

Author:

Bower Oliver J.¹,McCarthy Afshan¹,Lea Rebecca A.¹,Alanis‐Lobato Gregorio¹,Zohren Jasmin²,Gerri Claudia¹,Turner James M.A.²,Niakan Kathy K.¹³

Affiliation:

1. Human Embryo and Stem Cell Laboratory The Francis Crick Institute London United Kingdom

2. Sex Chromosome Biology Laboratory The Francis Crick Institute London United Kingdom

3. The Centre for Trophoblast Research, Department of Physiology, Development and Neuroscience University of Cambridge Cambridge United Kingdom

Publisher

Wiley

Subject

Medical Laboratory Technology,Health Informatics,General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/cpz1.232

Reference80 articles.

1. A global reference for human genetic variation

2. Large deletions induced by Cas9 cleavage

3. A short G1 phase imposes constitutive replication stress and fork remodelling in mouse embryonic stem cells

4. Pluripotent stem cells in disease modelling and drug discovery

5. N-terminal Truncation of Antiapoptotic MCL1, but Not G2/M-induced Phosphorylation, Is Associated with Stabilization and Abundant Expression in Tumor Cells

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