The Transpeptidase Sortase A Binds Nucleic Acids and Mediates Mammalian Cell Labeling

Author:

Liu Yingzheng1234,Lu Zhike1234,Wu Panfeng234,Liang Zhaohui5,Yu Zhenxing234,Ni Ke2345,Ma Lijia234ORCID

Affiliation:

1. College of Life Sciences Zhejiang University Hangzhou 310058 China

2. Westlake Laboratory of Life Sciences and Biomedicine 18 Shilongshan Road Hangzhou 310024 China

3. School of Life Sciences Westlake University 600 Dunyu Road Hangzhou 310030 China

4. Institute of Biology Westlake Institute for Advanced Study 18 Shilongshan Road Hangzhou 310024 China

5. AIdit Therapeutics 1 Yunmeng Road, Building 1 Hangzhou 310024 China

Abstract

AbstractWild‐type sortase A is an important virulence factor displaying a diverse array of proteins on the surface of bacteria. This protein display relies on the transpeptidase activity of sortase A, which is widely engineered to allow protein ligation and protein engineering based on the interaction between sortase A and peptides. Here an unknown interaction is found between sortase A from Staphylococcus aureus and nucleic acids, in which exogenously expressed engineered sortase A binds oligonucleotides in vitro and is independent of its canonical transpeptidase activity. When incubated with mammalian cells, engineered sortase A further mediates oligonucleotide labeling to the cell surface, where sortase A attaches itself and is part of the labeled moiety. The labeling reaction can also be mediated by many classes of wild‐type sortases as well. Cell surface GAG appears involved in sortase‐mediated oligonucleotide cell labeling, as demonstrated by CRISPR screening. This interaction property is utilized to develop a technique called CellID to facilitate sample multiplexing for scRNA‐seq and shows the potential of using sortases to label cells with diverse oligonucleotides. Together, the binding between sortase A and nucleic acids opens a new avenue to understanding the virulence of wild‐type sortases and exploring the application of sortases in biotechnology.

Publisher

Wiley

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