Affiliation:
1. Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School University of South China Hengyang China
2. NMPA Key Laboratory for Safety Evaluation of Cosmetics, Department of Hygiene Inspection & Quarantine Science, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health Southern Medical University Guangzhou China
3. Key Laboratory of Environment‐Friendly Chemistry and Application of Ministry of Education, Laboratory of Biochemistry, College of Chemistry Xiangtan University Xiangtan China
Abstract
AbstractFood contaminates, such as insecticide, may influence the toxicity of nanoparticles (NPs) to intestine. The present study investigated the combined toxicity of TiO2 NPs and fipronil to male mouse intestine. Juvenile mice (8 weeks) were orally exposed to 5.74 mg/kg TiO2 NPs, 2.5 mg/kg fipronil, or both, once a day, for 5 days. We found that both TiO2 NPs and fipronil induced some pathological changes in intestines, accompanying with defective autophagy, but these effects were not obviously enhanced after TiO2 NP and fipronil co‐exposure. Fipronil promoted Ti accumulation but induced minimal impact on other trace elements in TiO2 NP‐exposed intestines. Metabolomics data revealed that the exposure altered metabolite profiles in mouse intestines, and two KEGG pathways, namely, ascorbate and aldarate metabolism (mmu00053) and glutathione metabolism (mmu00480), were only statistically significantly changed after TiO2 NP and fipronil co‐exposure. Five metabolites, including 2‐deoxy‐D‐erythro‐pentofuranose 5‐phosphate, 5alpha‐cholestanol, beta‐D‐glucopyranuronic acid, elaidic acid, and isopentadecanoic acid, and maltotriose, were more significantly up‐regulated after the co‐exposure, whereas trisaccharide and xylonolactone were only significantly down‐regulated by the co‐exposure. We concluded that fipronil had minimal impact to enhance the toxicity of TiO2 NPs to mouse intestines but altered metabolite profiles.
Funder
Natural Science Foundation of Hunan Province