A simple method for expression and purification of Shiga toxin 1 (Stx1) with biological activities by using a single-promoter vector and native signal peptide-Reference-Cited by-同舟云学术

A simple method for expression and purification of Shiga toxin 1 (Stx1) with biological activities by using a single-promoter vector and native signal peptide

Published:2015-07-17 Issue:4 Volume:63 Page:539-545
ISSN:0885-4513
Container-title:Biotechnology and Applied Biochemistry
language:en
Short-container-title:Biotechnology and Applied Biochemistry

Author:

Tu Wei¹,Li Tao¹,Wang Qin¹,Cai Kun¹,Gao Xiang¹,Wang Hui¹

Affiliation:

1. State Key Laboratory of Pathogens and Biosecurity; Beijing Institute of Microbiology and Epidemiology; Fengtai District Beijing People's Republic of China

Funder

National Key Basic Research Program (973) of China

National Natural Science Foundation of China

National Science and Technology Major Project for Creation of Major New Drugs of China

State Key Laboratory of Pathogen and BioSecurity Program

Publisher

Wiley

Subject

Process Chemistry and Technology,Drug Discovery,Applied Microbiology and Biotechnology,Biomedical Engineering,Molecular Medicine,General Medicine,Bioengineering,Biotechnology

Link

http://onlinelibrary.wiley.com/wol1/doi/10.1002/bab.1398/fullpdf

Reference28 articles.

1. Furin-induced Cleavage and Activation of Shiga Toxin

2. Shiga-toxin-converting bacteriophages

3. Roles of a ribosome-binding site and mRNA secondary structure in differential expression of Shiga toxin genes

4. Nucleotide sequence analysis of the structural genes for Shiga-like toxin I encoded by bacteriophage 933J from Escherichia coli

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