A quantitative single-cell assay for retrograde membrane traffic enables rapid detection of defects in cellular organization-Reference-Cited by-同舟云学术

A quantitative single-cell assay for retrograde membrane traffic enables rapid detection of defects in cellular organization

Published:2020-03-19 Issue:7 Volume:31 Page:511-519
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Luong Phi¹²,Li Qian¹²³,Chen Pin-Fang⁴,Wrighton Paul J.⁵,Chang Denis¹²,Dwyer Sean⁴,Bayer Marie-Theres¹²,Snapper Scott B.¹²⁶,Hansen Steen H.¹²,Thiagarajah Jay R.¹²⁶,Goessling Wolfram⁷⁸⁹,Lencer Wayne I.¹²⁶

Affiliation:

1. Department of Pediatrics, Harvard Medical School, and

2. Division of Gastroenterology, Boston Children’s Hospital, Boston, MA 02115

3. Shanghai Children’s Hospital, Shanghai Jiaotong University, Shanghai 200000, China

4. F.M. Kirby Neurobiology Center, Translational Neuroscience Center, Boston Children’s Hospital and Harvard Medical School, Boston, MA 02115

5. Division of Genetics, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115

6. Harvard Digestive Disease Center, Harvard Medical School, Boston, MA 02115

7. Harvard Stem Cell Institute, Cambridge, MA 02138

8. Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115

9. Division of Gastroenterology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114

Abstract

Retrograde membrane trafficking from plasma membrane to the Golgi and endoplasmic reticulum affects intracellular protein dynamics underlying cell function. Here, we developed split-fluorescent toxin reporters that enable a quantitative, sensitive, and real-time single-cell flow cytometry assay for retrograde membrane transport.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

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