Affiliation:
1. Engineering Research Center of Bio-Process of Ministry of Education, H efei University of Technology Feicui road 420 Hefei 230601 China
2. School of Food and Biological Engineering Hefei University of Technology Feicui road 420 Hefei 230601 China
3. Anhui Key Laboratory of Modern Biomanufacturing Anhui University Jiulong Road 111 Hefei 230601 China
4. Huoshan County Changchong Medical Materials Development Co., Ltd Lu'an 237200 China
Abstract
AbstractThis study aimed to explore the alkaloid profile of Dendrobium huoshanense and determine the potential protective effect against oxidative damage. The crude D. huoshanense alkaloid extract (DHAE) was obtained by 70 % ethanol extraction and liquid‐liquid partition. DHAE contained specific alkaloid components with abundant 6‐hydroxynobiline (58.15 %) and trace dendrobine (3.23 %) in the preliminary HPLC fingerprint and GC‐MS analysis, which was distinguished from D. officinale or D. nobile. Subsequently, six alkaloids including 6‐hydroxynobiline, 2‐hydroxy dendrobine, nobilonine, dendrobine, Findlayines D and trans‐dendrochrysanine were identified in the purified DHAE (namely DHSAE‐3, DHSAE‐3′) via further solid phase extraction coupled with UPLC‐MS/MS analysis. Meanwhile, pretreatment with DHAE or DHSAE (0.5, 5 μg/mL) increased cell viability by 14.0–57.4 % compared to that of H2O2‐induced PC12 Model cells. Among them, 5 μg/mL DHSAE‐3‐treated cells displayed a pronounced reversion than the positive vitamin E (p<0.01). Furthermore, a clear cellular morphological restoration and 38.4 % reduction in intracellular reactive oxidative species level were achieved. Our findings suggest that D. huoshanense has a characteristic alkaloid profile represented by abundant 6‐hydroxynobiline, and DHAEs exhibit obvious protection against oxidative neuronal damage. Overall, this study indicates that DHAEs might be used to inhibit oxidative stress and provide a source to develop novel neuroprotective drugs.
Subject
Molecular Biology,Molecular Medicine,General Chemistry,Biochemistry,General Medicine,Bioengineering