Affiliation:
1. National key Laboratory of Non-food Biomass Energy Technology National Engineering Research Center for Non-Food Biorefinery Guangxi Academy of Sciences 98 Daling Road Nanning 530007 China
2. Life Science and Technology College State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi University Nanning 530004 China
Abstract
AbstractMicrobial endophytes are known as versatile producers of useful metabolites, which have extensive applications in pharmacy, fragrance, agriculture and food. This study aims to screen sclareol‐biotransforming microorganisms from Salvia sclarea, an untapped source of diverse endophytes. In this study, 50 culturable endophytes were isolated from S. sclarea grown in Xinjiang using sclareol as the sole carbon source and screened for their potential to transform sclareol into analogues. A fungal endophyte, identified as the generally recognized as safe (GRAS) strain Aspergillus tubingensis, can produce labd‐14‐ene‐3β,8α,13β‐triol and 8α,13β‐dihydroxylabd‐14‐en‐3‐one from sclareol, involving hydroxylation and carbonylation at the C3 site. Structures of the two metabolites were elucidated by HR‐ESI‐MS and NMR analysis. S. sclarea was proven to be a good source of endophytes that are prospective producers of secondary metabolites with valuable chemical and biological properties. This study is the first report regarding the isolation of endophytes from S. sclarea.
Funder
Science and Technology Major Project of Guangxi
Subject
Molecular Biology,Molecular Medicine,General Chemistry,Biochemistry,General Medicine,Bioengineering