Strain engineering to prevent norleucine incorporation during recombinant protein production inEscherichia coli

Author:

Veeravalli Karthik1,Laird Michael W.1,Fedesco Mark2,Zhang Yu3,Yu X. Christopher3

Affiliation:

1. Dept. of Late Stage Cell Culture; Genentech Inc.; South San Francisco CA 94080

2. Dept. of Purification Development; Genentech Inc.; South San Francisco CA 94080

3. Dept. of Protein Analytical Chemistry; Genentech Inc.; South San Francisco CA 94080

Publisher

Wiley

Subject

Biotechnology

Reference29 articles.

1. Identification of codon-specific serine to asparagine mistranslation in recombinant monoclonal antibodies by high-resolution mass spectrometry;Yu;Anal Chem,2009

2. G/U and certain wobble position mismatches as possible main causes of amino acid misincorporations;Zhang;Biochemistry,2013

3. Biosynthesis and incorporation into protein of norleucine by Escherichia coli;Bogosian;J Biol Chem,1989

4. Overproduction of noncanonical amino acids by Escherichia coli cells;Sycheva;Mikrobiologiia,2007

5. Control of misincorporation of de novo synthesized norleucine into recombinant interleukin-2 in E;Tsai;coli. Biochem Biophys Res Commun,1988

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