Affiliation:
1. AcSIR-Academy of Scientific and Innovative Research, New Delhi, India
2. Medicinal, Aromatic and Economic Plants Group, Biological Science and Technology Division, CSIR-North East Institute of Science and Technology (NEIST), Jorhat, Assam-785006, India
3. Medicinal, Aromatic and Economic Plants Group, Biological Science and Technology Division CSIR-North East Institute of Science and Technology (NEIST), Jorhat, Assam-785006, India
Abstract
Background:
This investigation was designed to evaluate the chemical composition, antioxidant,
anti-inflammatory, genotoxicity, and antimicrobial activities of Curcuma caesia Roxb rhizome
essential oil.
Methods:
Gas Chromatography/Mass Spectroscopy (GC/MS) analysis was performed to determine the
chemical composition, standard antioxidative test DPPH assay, reducing power assay, in vitro antiinflammatory
activity (egg albumin denaturation, protease inhibitory assay) by using standard methods.
Similarly, antimicrobial activity was tested using the disc diffusion method, minimum inhibitory
concentration ability (MIC); while to test genotoxicity, Allium cepa assay was used.
Results:
GC/MS analysis revealed eucalyptol (28.55%), epicurzerenone (19.62%), and camphor
(21.73%) as the major components of C. caesia rhizome essential oil. Potent antioxidant (IC50=
48.08±0.003 μg/mL), anti-inflammatory (IC50= 121.7±0.0013 μg/mL), and antimicrobial activities of
the essential oil were recorded better than the standard drugs Fluconazole for fungus and Ciprofloxacin
for bacteria. The essential oil also possessed a strong antibacterial effect against two tested bacterial
strains B. subtilis and B. cereus with 7.5 μg/mL MIC value, while for fungal strains the essential oil
was most effective against S. cereviaceae with an MIC value of 2.5 μg/mL. All the data were recorded
in triplicates. Allium cepa assay revealed minor genotoxicity with mitotic index, MI= 27.70%; chromosome
aberration, A= 1.1% of C. caesia rhizome essential oil.
Conclusion:
C. caesia rhizome essential oil possesses potent antioxidant, anti-inflammatory, and antimicrobial
properties with negligible genotoxicity. Hence, the present study is highly significant for the
utilization of rhizome of C. caesia, a high-value ethnopharmacological plant for advanced R & D and
commercial application.
Funder
National Medicinal Plants Board, Ministry of AYUSH, Government of India
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmaceutical Science,Biotechnology
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