Antioxidant, Anti-Inflammatory, and Anti-Bacterial Activities of Artemisia vulgaris L. Essential Oil in Vietnam

Abstract

Background Artemisia vulgaris L. (AV) is a beneficial herb with therapeutic properties. This work aims to evaluate the compositions and bioactivity of essential oil from AV grown in Tiengiang, Vietnam. Methods The essential oils (AVEO) were extracted by hydrodistillation (HD), and the headspace volatiles (HS) were collected using the static headspace technique. Gas chromatography/mass spectrometry (GC/MS) was used to examine the compositions of oils in detail. The agar well-diffusion technique was used to conduct the antibacterial test. Multi-concentration dilution method was used for minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determination. The cell viability was determined by the MTT method. Nitric oxide and TNF-α in RAW 264.7 were analyzed by Griess reagent or ELISA as described by the manufacturer. In addition, DPPH, ABTS, and reducing power assay were used to determine the antioxidant activity. Results The HD method yielded 1,8-cineole, α-pinene, β -caryophyllene, borneol, camphor, and δ-elemene were the most abundant components. In contrast, the HS approach produced the primary constituents being camphor, 2-methylbutanal, 1,8-cineole, and camphene. The HD showed antibacterial activities against E.coli, S.typhimurium, S .aureus, P.aeruginosa, and S.mutans, and inhibited the production of NO, TNF-α in lipopolysaccharide-induced RAW264.7. Furthermore, the HD indicated moderate antioxidant activity. A hierarchical cluster analysis of essential oils from 22 regions shows that mugwort oils in Tiengiang, Vietnam, have a high level of δ-elemene (more than 5%), which is not common in oils from other species. The study's findings enhance our comprehension of the chemical components and chemical variety of mugwort oil across different sites and to help in the identification of the predominant species suitable for extracting essential oils for different purposes within the Artemisia vulgaris L. species. Conclusion These findings collectively suggest that essential oils derived from A. vulgaris have a range of potential therapeutic uses.