Heritable Custom Genomic Modifications in Caenorhabditis elegans via a CRISPR

Heritable Custom Genomic Modifications in Caenorhabditis elegans via a CRISPR–Cas9 System

Published:2013-11-01 Issue:3 Volume:195 Page:1181-1185
ISSN:1943-2631
Container-title:Genetics
language:en
Short-container-title:

Author:

Tzur Yonatan B¹,Friedland Ari E¹,Nadarajan Saravanapriah¹,Church George M¹²,Calarco John A³,Colaiácovo Monica P¹

Affiliation:

1. Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115

2. Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts 02138

3. FAS Center for Systems Biology, Harvard University, Cambridge, Massachusetts 02138

Abstract

Abstract We adapted the CRISPR–Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific insertions, deletions, and base pair changes in the germline of C. elegans.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

https://academic.oup.com/genetics/article-pdf/195/3/1181/42116203/genetics1181.pdf

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