Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins-Reference-Cited by-同舟云学术

Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins

Published:2013-11-01 Issue:3 Volume:195 Page:1177-1180
ISSN:1943-2631
Container-title:Genetics
language:en
Short-container-title:

Author:

Cho Seung Woo¹,Lee Jihyun²,Carroll Dana³,Kim Jin-Soo¹,Lee Junho²⁴

Affiliation:

1. Department of Chemistry, Seoul National University, Seoul 151-742, Korea

2. Department of Biological Sciences, Seoul National University, Seoul 151-742, Korea

3. Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112-5650

4. Department of Biophysics and Chemical Biology, Seoul National University, Seoul 151-742, Korea

Abstract

Abstract We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F1 progeny, demonstrating the high efficiency of this method.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

https://academic.oup.com/genetics/article-pdf/195/3/1177/42116223/genetics1177.pdf

Reference24 articles.

1. Cuticle surface proteins of wild type and mutant Caenorhabditis elegans.;Blaxter;J. Biol. Chem.,1993

2. UNC-1 regulates gap junctions important to locomotion in C. elegans.;Chen;Curr. Biol.,2007

3. Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas.;Chiu;Genetics,2013

4. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.;Cho;Nat. Biotechnol.,2013

Cited by 236 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Single molecule delivery into living cells;2024-01-09

2. Nonsense-mediated decay machinery in Plasmodium falciparum is inefficient and non-essential;mSphere;2023-08-24

3. Colour change in potato (Solanum tuberosum L.) tubers by disruption of the anthocyanin pathway via ribonucleoprotein complex delivery of the CRISPR/Cas9 system;2023-08-18

4. Efficient CRISPR/Cas9 mediated large insertions using long single‐stranded oligonucleotide donors in C. elegans;The FEBS Journal;2023-06-08

5. CRISPR/Cas9 mediated gene editing in non-model nematode Panagrolaimus sp. PS1159;Frontiers in Genome Editing;2023-02-03