Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas-Reference-Cited by-同舟云学术

Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas

Published:2013-11-01 Issue:3 Volume:195 Page:1167-1171
ISSN:1943-2631
Container-title:Genetics
language:en
Short-container-title:

Author:

Chiu Hui¹²,Schwartz Hillel T¹²,Antoshechkin Igor¹,Sternberg Paul W¹²

Affiliation:

1. Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California 91125

2. Howard Hughes Medical Institute, Pasadena, California 91125

Abstract

Abstract CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates that the nuclease activity is highly specific.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

https://academic.oup.com/genetics/article-pdf/195/3/1167/42119538/genetics1167.pdf

Reference28 articles.

1. Mobilization of a Drosophila transposon in the Caenorhabditis elegans germ line.;Bessereau;Nature,2001

2. Heritable gene knockout in Caenorhabditis elegans by direct injection of Cas9-sgRNA ribonucleoproteins;Cho;Genetics,2013

3. Multiplex genome engineering using CRISPR/Cas systems.;Cong;Science,2013

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