Effects of dietary Euphorbia heterophylla extract on the growth performance, physiological, antioxidative and immune responses of Clarias gariepinus juveniles

Abstract

AbstractEffects of dietary Euphorbia heterophylla extract (EH) on growth performance, feed utilization and haemato‐biochemical parameters in African catfish, Clarias gariepinus, juveniles were evaluated in this study. Diets fortified with EH at 0 (control), 0.5, 1.0, 1.5 or 2.0 g/kg were fed to the fish to apparent satiation for 84 days before challenging it with Aeromonas hydrophila. The weight gain, specific growth rate and protein efficiency ratio of fish fed EH‐supplemented diets were significantly higher but lower feed conversion ratio (p < 0.05) than the control group. The villi height and width at the proximal, mid and the distal of the guts rose significantly with the increasing levels of EH from 0.5 to 1.5 g than the fish fed basal diet. Dietary EH enhanced (p < 0.05) the packed cell volume and haemoglobin, whereas 1.5 g EH boosted white blood cell, in relation to their counterpart in the control group. There were significant increase in the activities of glutathione‐S‐transferase, glutathione peroxidase and superoxide dismutase (p < 0.05) in the fish that were fed diets supplemented with EH than the control. Dietary EH also enhanced phagocytic activities, lysozyme activities and relative survival (RS) of C. gariepinus than the control group, with the highest RS obtained in fish that were fed diet containing EH at 1.5 g/kg level. These results revealed that the fish fed 1.5 g/kg dietary EH promoted growth performance, antioxidant and immune profiles, as well as protection against A. hydrophila infection.