Determining predictive metabolomic biomarkers of meniscal injury in dogs with cranial cruciate ligament rupture

Abstract

ObjectivesThis study used hydrogen nuclear magnetic resonance spectroscopy for the first time to examine differences in the metabolomic profile of stifle joint synovial fluid from dogs with cranial cruciate ligament rupture with and without meniscal injuries, in order to identify biomarkers of meniscal injury. Identifying a biomarker of meniscal injury could then ultimately be used to design a minimally invasive diagnostic test for meniscal injuries in dogs.Materials and MethodsStifle joint synovial fluid was collected from dogs undergoing stifle joint surgery or arthrocentesis for lameness investigations. We used multi‐variate statistical analysis using principal component analysis and univariate statistical analysis using one‐way analysis of variance and analysis of co‐variance to identify differences in the metabolomic profile between dogs with cranial cruciate ligament rupture and meniscal injury, cranial cruciate ligament rupture without meniscal injury, and neither cranial cruciate ligament rupture nor meniscal injury, taking into consideration clinical variables.ResultsA total of 154 samples of canine synovial fluid were included in the study. Sixty‐four metabolites were annotated to the hydrogen nuclear magnetic resonance spectroscopy spectra. Six spectral regions were found to be significantly altered (false discovery rate adjusted P‐value <0.05) between groups with cranial cruciate ligament rupture with and without meniscal injury, including three attributed to nuclear magnetic resonance mobile lipids [mobile lipid ‐CH3 (P=0.016), mobile lipid ‐n(CH3)3 (P=0.017), mobile unsaturated lipid (P=0.031)].Clinical SignificanceWe identified an increase in nuclear magnetic resonance mobile lipids in the synovial fluid of dogs with meniscal injury which are of interest as potential biomarkers of meniscal injury.