Antimutagenic constituents from Monanthotaxis caffra (Sond.) Verdc.

Author:

Makhuvele Rhulani12,Foubert Kenn3,Apers Sandra3,Pieters Luc3,Verschaeve Luc45,Elgorashi Esameldin12ORCID

Affiliation:

1. Toxicology and Ethnoveterinary Medicine, ARC-Onderstepoort Veterinary Institute, Onderstepoort, South Africa

2. Department of Paraclinical Sciences, University of Pretoria, Onderstepoort, South Africa

3. Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Antwerp-Wilrijk, Belgium

4. Toxicology, Scientific Institute of Public Health, Brussels, Belgium

5. Department of Biomedical Sciences, University of Antwerp, Antwerp-Wilrijk, Belgium

Abstract

Abstract Objectives Monanthotaxis caffra (Sond.) Verdc. (Annonaceae) has been reported to possess antitumoural properties. Preliminary screening showed that the crude methanolic leaf extract had strong antimutagenic effects against aflatoxin B1-induced mutagenicity. The aim of this study was to isolate and evaluate the antimutagenic properties of the active constituents from M. caffra. Methods Different chromatographic, spectroscopic and spectrometric techniques were used for the isolation and identification of the antimutagenic constituents. The antimutagenic effect of the extract and compounds was evaluated using Ames, Vitotox and Comet assays. Key findings Bioassay-guided fractionation of the methanolic leaf extract yielded two antimutagenic compounds identified as (+)-crotepoxide and 5,6-diacetoxy1-benzoyloxymethyl-1,3-cyclohexadiene. Crotepoxide had strong antimutagenicity in the Vitotox assay with an IC50 value of 131 μg/ml. 5,6-Diacetoxy-1-benzoyloxymethyl-1,3-cyclohexadiene showed strong antimutagenic activity in the Ames assay with an IC50 value of 348.9 μg/plate and no antimutagenic activity in the Vitotox test. Furthermore, the compound was able to inhibit, block or prevent biotransformation of aflatoxin B1 by repressing the proteins involved in transcription. Conclusions Crotepoxide and 5,6-diacetoxy-1-benzoyloxymethyl-1,3-cyclohexadiene have the potential to mitigate the risks arising from consumption of aflatoxin B1-contaminated food and feed.

Funder

National Research Foundation

Fonds Wetenschappelijk Onderzoek

Flemish Interuniversity Council

Publisher

Oxford University Press (OUP)

Subject

Pharmaceutical Science,Pharmacology

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