Abstract
Introduction. The use of traditional medicinal plant concoctions to cure or treat different diseases daily in African folk medicine. However, the effects of most medicinal plants on human health or genetic material remain unknown. This study thus aimed to evaluate the mutagenic and antimutagenic potentials of Asparagus laricinus Burch. cladodes, Senecio asperulus DC., and Gunnera perpensa L. roots extract in vitro.
Methods. Neutral red uptake assay, alkaline comet assay, and the VITOTOX test was used with plant extract dilutions of 4, 20, 50, and 100 µg/ml, respectively, on hepatic (C3A) cells and Salmonella Typhimurium TA104 strains. Ethyl methane-sulfonate and 4-nitroquinoline oxide were used as positive controls for the comet and VITOTOX assays, respectively.
Results. In vitro cytotoxicity and genotoxicity were not observed from all tested extracts, except for the two dichloromethane (DCM) extracts of S. asperulus and G. perpensa, which appeared to be cytotoxic with S9 metabolic activation, but not genotoxic or mutagenic. From the VITOTOX test results, none of the extracts appeared to have antimutagenic properties after treating S. Typhimurium strains with a known mutagen.
Conclusions. These results confirm that previously reported anticarcinogenic properties of A. laricinus, S. asperulus, and G. perpensa did not result from the protective mechanism against genotoxicity but from other ones. Moreover, the negative mutagenic and cytotoxic activities of the tested plants highlighted the safe use of these medicinal plants in vitro. Therefore, S. asperulus and G. perpensa DCM extracts require further investigation for their possible in vivo cytotoxic effects on humans.
Publisher
Poznan University of Medical Sciences
Reference44 articles.
1. Abd Razak, M, F., K.E. Aidoo and A.G. Candlish, 2007. Mutagenic and cytotoxic properties of three herbal plants from Southeast Asia. Trop Biomed, 24(2): 49-59. https://europepmc.org/article/med/18209708
2. Roy, A., T. Attre and N. Bharadvaja, 2017. Anticancer agent from medicinal plants: a review. In book: New aspects in medicinal plants and pharmacognosy, 1st edition.;1(3): 54-73. https://www.researchgate.net/profile/Arpita-Roy-3/publication/318721809_Anticancer_agent_from_medicinal_plants_a_review/links/597ace074585151e35aea568/Anticancer-agent-from-medicinal-plants-a-review.pdf
3. Verschaeve, L., H. Edziri, R. Anthonissen, D. Boujnah, F. Skhiri and H Chehab, 2017. In vitro toxicity and genotoxic activity of aqueous leaf extracts from four varieties of Olea europea (L). Pharmacog Magazine, 13(S1): S63-S80. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5407118/
4. Verschaeve, L., J. Van Gompel, L. Thilemans, L. Regniers, P. Vanparys and D. Van der Lelie, 1999. VITOTOX® bacterial genotoxicity and toxicity test for the rapid screening of chemicals. Environ and Mol Mutagen, 33(3): 240-8. https://onlinelibrary.wiley.com/doi/abs/10.1002/(SICI)1098-2280(1999)33:3%3C240::AID-EM8%3E3.0.CO;2-R
5. Griffiths, A.J., J.H. Miller, D.T. Suzuki, R.C. Lewontin and W.M. Gelbart, 2000. Quantifying heritability. In Freeman WH, ed. An Introduction to Genetic Analysis, 7th edition.. http://lgb.rc.unesp.br/biomol/literatura/Griffiths_8th.pdf