Affiliation:
1. Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science Seoul National University Seoul Republic of Korea
2. Department of Nano-Bio Convergence Korea Institute of Materials Science Changwon Republic of Korea
3. Institute of Green Bio Science and Technology Seoul National University Pyeongchang South Korea
Abstract
Abstract
Aim
This study aimed to examine the inactivation efficacy of plasma-activated acetic acid (PAAA) against Salmonella Typhimurium cells and biofilm and elucidate underlying chemical inactivation pathway.
Methods and Results
PAAA was prepared by discharging plasma to 20 ml of 0.2% (v/v) acetic acid (AA) for 20 min (2.2 kHz and 8.4 kVpp). The count of cells and biofilms decreased by 5.71 log CFU ml−1 and 4 log CFU/cm2 after 10 min of treatment with 0.2% PAAA and 0.4% PAAA compared with control group (without any treatment), respectively. In 0.2% PAAA, the concentrations of hydrogen peroxide (H2O2) and nitrate anions were directly proportional to the plasma discharge time, whilst nitrite anion (NO2−) was not detected. However, the pH values of both 0.2% PAAA and plasma-activated water were inversely proportional to the plasma discharge time. Treatment with catalase, L-histidine, D-mannitol and sodium azide inhibited the antibacterial activity of PAAA.
Conclusion
H2O2, singlet oxygen, hydroxyl radical and NO2− are involved in the generation and decomposition of peroxynitrous acid generated from PAAA functioned as intermediate agent, which could diffuse through cell membranes of bacteria and induce cell injury.
Significance and Impact of Study
This study provides the understanding of efficacy and selectivity of PAAA which could be a novel decontamination agent.
Funder
Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,General Medicine,Biotechnology
Cited by
4 articles.
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