A prolific and robust whole‐genome genotyping method using PCR amplification via primer‐template mismatched annealing

Author:

Zhao Sheng12,Zhang Cuicui2,Wang Liqun1,Luo Minxuan12,Zhang Peng2,Wang Yue2,Malik Waqar Afzal2,Wang Yue2,Chen Peng2,Qiu Xianjin3,Wang Chongrong4,Lu Hong2,Xiang Yong2,Liu Yuwen2,Ruan Jue2,Qian Qian2,Zhi Haijian1,Chang Yuxiao2

Affiliation:

1. National Key Laboratory for Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement Nanjing Agricultural University Nanjing 210095 China

2. Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518120 China

3. College of Agriculture Yangtze University Jingzhou 434023 China

4. Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Rice Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 China

Publisher

Wiley

Subject

Plant Science,General Biochemistry, Genetics and Molecular Biology,Biochemistry

Reference34 articles.

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2. Trimmomatic: a flexible trimmer for Illumina sequence data

3. Chang Y.X. Liu K. Zhao S. Zhang H. andWang Q.(2018). A method for isolating unknown flanking sequence of multiple known DNA fragments simultaneously. China Patent ZL201811171982.5.

4. Twelve years of SAMtools and BCFtools

5. RNA sequencing by direct tagmentation of RNA/DNA hybrids

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