Genetic Diversity and Population Structure Analysis of Hollyhock (Alcea rosea Cavan) Using High-Throughput Sequencing

Author:

Wang Yue12,Zhao Sheng2ORCID,Chen Peng2,Liu Yongkang2ORCID,Ma Zhigang2,Malik Waqar Afzal2,Zhu Zhenghang2,Peng Zhenyu34,Lu Haorong34,Chen Yanli1,Chang Yuxiao2

Affiliation:

1. Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Sanya Nanfan Research Institute of Hainan University, School of Horticulture, Hainan University, Haikou 570228, China

2. Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China

3. BGI-Shenzhen, Shenzhen 518083, China

4. China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China

Abstract

Hollyhock (Alcea rosea (Linn). Cavan) is an herbaceous flowering plant with significant applications in urban greening, soil remediation, and traditional medicine. However, its genetic diversity and molecular characteristics at the population level have not been explored yet. Here, the phenotypic and genetic diversity of 162 hollyhock accessions from China revealed extensive variation among 11 traits and strong correlations between several quantitative traits. Whole-genome re-sequencing of 32 randomly chosen accessions identified 10,468,760 core single-nucleotide polymorphisms (SNPs) distributed evenly across the genome, except for on chromosome 21, and the average nucleotide diversity (π) was calculated to be 0.00397. Principal component analysis showed that these 32 accessions could be divided into four subpopulations, which was in agreement with the population structure analysis, and the subpopulations were strongly correlated with geographic location. A neighbor-joining dendrogram displayed similar clusters, except for accessions HuB25 and HLJ28, which formed two separate clusters. Our findings illuminate the genetic diversity in hollyhock and provide valuable information for hollyhock breeding.

Funder

R&D program of Shenzhen

R&D program in key areas of Guangdong Province

Science and Technology Planning Project of Guangdong Province

Hainan Major Science and Technology Project

Hainan Province Science and Technology Special Fund

Publisher

MDPI AG

Subject

Horticulture,Plant Science

Reference57 articles.

1. Effects of intercropping ryegrass with hollyhock and inoculation with Bacillus thuringiensis on enhancing phytoremediation of Cd-contaminated soils;Chen;Chemosphere,2023

2. Muhammad, A.H., Haq, N., Muhammad, M.K., and Hugh, J.B. (2019). Medicinal Plants of South Asia: Novel Sources for Drug Discovery, Elsevier. [1st ed.].

3. Li, S. (1982). Compendium of Materia Medica, People’s Medical Publishing House.

4. Callogenesis potential of cotyledonary explants of Althaea rose L. from Pakistan;Munir;Pak. J. Bot.,2012

5. A Study of the Paintings of Xixia Grottoes in Dunhuang on the background of art history;Wang;J. Dunhuang Stud.,2019

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