The receptor kinase RiSho1 in Rhizophagus irregularis regulates arbuscule development and drought tolerance during arbuscular mycorrhizal symbiosis

Author:

Wang Sijia1ORCID,Han Lina1ORCID,Ren Ying1ORCID,Hu Wentao1ORCID,Xie Xianan1ORCID,Chen Hui1ORCID,Tang Ming1ORCID

Affiliation:

1. State Key Laboratory of Conservation and Utilization of Subtropical Agro‐Bioresources, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture South China Agricultural University Guangzhou 510642 China

Abstract

Summary In terrestrial ecosystems, most plant species can form beneficial associations with arbuscular mycorrhizal (AM) fungi. Arbuscular mycorrhizal fungi benefit plant nutrient acquisition and enhance plant tolerance to drought. The high osmolarity glycerol 1 mitogen‐activated protein kinase (HOG1‐MAPK) cascade genes have been characterized in Rhizophagus irregularis. However, the upstream receptor of the HOG1‐MAPK cascade remains to be investigated. We identify the receptor kinase RiSho1 from R. irregularis, containing four transmembrane domains and one Src homology 3 (SH3) domain, corresponding to the homologue of Saccharomyces cerevisiae. Higher expression levels of RiSho1 were detected during the in planta phase in response to drought. RiSho1 protein was localized in the plasma membrane of yeast, and interacted with the HOG1‐MAPK module RiPbs2 directly by protein–protein interaction. RiSho1 complemented the growth defect of the yeast mutant ∆sho1 under sorbitol conditions. Knock‐down of RiSho1 led to the decreased expression of downstream HOG1‐MAPK cascade (RiSte11, RiPbs2, RiHog1) and drought‐resistant genes (RiAQPs, RiTPSs, RiNTH1 and Ri14‐3‐3), hampered arbuscule development and decreased plants antioxidation ability under drought stress. Our study reveals the role of RiSho1 in regulating arbuscule development and drought‐resistant genes via the HOG1‐MAPK cascade. These findings provide new perspectives on the mechanisms by which AM fungi respond to drought.

Funder

National Natural Science Foundation of China

Publisher

Wiley

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