STUDIES ON THE LIVETINS OF HEN'S EGG YOLK: I. IDENTIFICATION OF PAPER-ELECTROPHORETIC AND IMMUNOELECTRO-PHORETIC LIVETIN FRACTIONS WITH SERUM PROTEIN ANTIGENS BY IMMUNOELECTROPHORETIC ANALYSIS

Abstract

Immunoelectrophoretic analysis of an egg yolk livetin preparation has demonstrated the presence of six distinct fractions, which were numbered 1, 2, 3, 4, 5, and 6 from the anodic end of the electropherogram. Four of these fractions have been identified with four livetin fractions obtained by paper electrophoresis as follows: immunoelectrophoretic fraction 1 with alpha-livetin, fraction 2 with beta-livetin, fraction 4 with gamma1-livetin, and fraction 5 with gamma2-livetin.Immunoelectrophoresis of hen serum or cock serum against antilivetin serum has shown that both hen and cock sera contain antigens electrophoretically and immunologically identical to alpha-, beta-, gamma1-, and gamma2-livetins.