A novel multiplex PCR assay to detect and distinguish between different types of Paenibacillus larvae and Melissococcus plutonius, and a survey of foulbrood pathogen contamination in Japanese honey-Reference-Cited by-同舟云学术

A novel multiplex PCR assay to detect and distinguish between different types of Paenibacillus larvae and Melissococcus plutonius, and a survey of foulbrood pathogen contamination in Japanese honey

Published:2022 Issue:3 Volume:84 Page:390-399
ISSN:0916-7250
Container-title:Journal of Veterinary Medical Science
language:en
Short-container-title:The Journal of Veterinary Medical Science

Author:

OKAMOTO Mariko¹,FURUYA Hirotaka²,SUGIMOTO Ikuko³,KUSUMOTO Masahiro⁴,TAKAMATSU Daisuke⁵

Affiliation:

1. Division of Infectious Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan

2. Gunma Livestock Health Laboratory, Gunma, Japan

3. Shizuoka Prefectural Chubu Livestock Hygienic Service Center, Shizuoka, Japan

4. Division of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan

5. The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan

Publisher

Japanese Society of Veterinary Science

Subject

General Veterinary

Link

https://www.jstage.jst.go.jp/article/jvms/84/3/84_21-0629/_pdf

Reference58 articles.

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2. 2. Antúnez, K., D’Alessandro, B., Piccini, C., Corbella, E. and Zunino, P. 2004. Paenibacillus larvae larvae spores in honey samples from Uruguay: a nationwide survey. J. Invertebr. Pathol. 86: 56–58.

3. 3. Arai, R., Miyoshi-Akiyama, T., Okumura, K., Morinaga, Y., Wu, M., Sugimura, Y., Yoshiyama, M., Okura, M., Kirikae, T. and Takamatsu, D. 2014. Development of duplex PCR assay for detection and differentiation of typical and atypical Melissococcus plutonius strains. J. Vet. Med. Sci. 76: 491–498.

4. 4. Arai, R., Tominaga, K., Wu, M., Okura, M., Ito, K., Okamura, N., Onishi, H., Osaki, M., Sugimura, Y., Yoshiyama, M. and Takamatsu, D. 2012. Diversity of Melissococcus plutonius from honeybee larvae in Japan and experimental reproduction of European foulbrood with cultured atypical isolates. PLoS One 7: e33708.

5. 5. Bailey, L. and Ball, B. V. 1991. Honey Bee Pathology, Academic Press, London.

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